High-gravity brewing utilizing factorial design

High-Gravity Brewing: Influence of High-Ethanol Beer on the Viability of Contaminating Brewing Bacteria

It has been reported that when high-gravity brewers' worts were supplemented with a source of nitrogen and unsaturated lipids and sterol, ethanol concentrations up to 16.4% v/v could be achieved within normal fermentation times. As the resultant harvested yeast can be repitched over a number of generations, there appears to be no reason in industry to limit the gravities of commercial worts to ...

Genome Sequence of the Lager-Brewing Yeast Saccharomyces sp. Strain M14, Used in the High-Gravity Brewing Industry in China

Lager-brewing yeasts are mainly used for the production of lager beers. Illumina and PacBio-based sequence analyses revealed an approximate genome size of 22.8 Mb, with a GC content of 38.98%, for the Chinese lager-brewing yeast Saccharomyces sp. strain M14. Based on ab initio prediction, 9,970 coding genes were annotated.

Using high pitching rate for improvement of yeast fermentation performance in high gravity brewing

One of the promising methods to improve yeast fermentation performance in high gravity brewing is to increase pitching rate in the bioreactor. In this study, fermentation with four different pitching rates (1.5×107, 4.5×107, 7.5×107, and 10.5×107 viable cells/ml wort) was carried out to investigate the impact of pitching rate on yeast fermentation performance and beer quality from 24oBx wort. T...

Factorial Design

Using fed-batch fermentation in high-gravity brewing: effects of nutritional supplementation on yeast fermentation performance

To intensify the brewing process and increase the fermenter productivity, many breweries throughout the world have been embracing high-gravity brewing. However, increasing original wort concentration can cause a negative effect on yeast fermentation performance. This study focused on the application of fed-batch fermentation in high-gravity brewing. High gravity worts of 24oBx (using 30% high m...